Review

recombinant mouse il 13 (R&D Systems)

R&D Systems is a verified supplier

R&D Systems manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

R&D Systems recombinant mouse il 13
Recombinant Mouse Il 13, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il+13/Recombinant+Mouse+IL-13+Protein/pm41708397-84-14-17
Average 95 stars, based on 90 article reviews

recombinant mouse il 13 - by Bioz Stars, 2026-09

95/100 stars

Images

Recombinant:

Article Title: Early growth response 2 as a target of IL-13/STAT6 signaling induces the goblet cell metaplasia of allergic airway epithelia.
Article Snippet: .. 2 h after adenoviral aerosolization, mice were further intratracheally aerosolized with 50 μl/mouse of recombinant mouse IL-13 (R&D Systems, Inc., Minneapolis, MN) at 100 μg/ml or NS as a control, once every other day for 5 consecutive days (from day 1 to day 5). ..

Article Title: LncRNA-MM2P regulates retinal neovascularization through M2 macrophage polarization.
Article Snippet: The study aims to investigate the effects and potential mechanisms of lncRNA-MM2P on retinal neovascularization in a mouse model of oxygen-induced retinopathy (OIR).. The OIR model was established in C57BL/6J mice.. RAW264.7 cell line and bone marrow-derived macrophages (BMDMs) from mice were used for in vitro studies.

Article Title: IL-13 Controls IL-33 Activity through Modulation of ST2.
Article Snippet: .. NIH/3T3 mouse embryonic fibroblasts (American Type Culture Collection, Manassas, VA) were plated at 2 105 cells/well in a 12-well plate and stimulated with recombinant mouse IL-13 (R&D Systems) or IL-33 for 22 h at maximal concentrations of 25 and 180 ng/ml respectively, with 10-fold serial dilutions. ..

Article Title: IL-13 Controls IL-33 Activity through Modulation of ST2.
Article Snippet: .. For in vitro assays, fibroblasts were plated at 3 105 to 8 105 cells/well and stimulated with recombinant mouse IL-13 or IL-4 (R&D Systems) for 24 h at maximal concentrations of 250 ng/ml with 10-fold serial dilutions. ..

Article Title: Interleukin 13 signaling modulates dopaminergic functions and nicotine reward in rodents
Article Snippet: .. Recombinant mouse IL-13 (Cat# 413-ML), IL-4 (Cat# 404-ML), IL-2 (Cat# 402-ML) were purchased from R&D Systems. ..

Article Title: Preadipocyte IL-13/IL-13R α 1 signaling regulates beige adipogenesis through modulation of PPAR γ activity
Article Snippet: .. Preadipocytes were plated at a density of 5 × 10 4 cells per well (12-well plates) and left overnight, followed by treatment with 10 ng/mL recombinant mouse IL-13 (or IL-4, R&D Systems), 1 μM Rosi (MilliporeSigma), or a combination of IL-13 (10 ng/mL) plus Rosi (1 μM) for 24 hours. .. The p38-MAPK inhibitor SB203580 (Abcam), the STAT6i AS1517499 (Selleck Chemicals), and the STAT3i Stattic (Abcam) were suspended in DMSO and administered at a concentration of 10 μM and 1 μM, respectively.

Article Title: Dihydroartemisinin inhibits Lewis Lung carcinoma progression by inducing macrophages M1 polarization via AKT/mTOR pathway.
Article Snippet: Preclinical and clinical data show a close relationship between high infiltration of tumor-associated macrophages (TAMs) and a poor prognosis in most types of tumors, thus targeting TAMs stands out as promising anticancer immunotherapies.. Recent studies have demonstrated the anti-tumor effects of artemisinin via enhancing antitumor immunity within tumor microenvironment, but the underlying mechanism is still not clear.. In the present study we uncovered an important role of dihydroartemisinin (DHA) in regulating intratumoral TAM polarization and anti-tumor immune responses in mouse Lewis Lung carcinoma model. We found that DHA inhibited Lewis Lung carcinoma progress, moderately decreased the frequencies of TAMs within tumor stroma, and significantly increased CD86 expression while decreased CD206 expression on TAMs which indicates the role of DHA in polarizing TAMs into a M1-like phenotype.

Article Title: Interleukin 13 signaling modulates dopaminergic functions and nicotine reward in rodents.
Article Snippet: .. Recombinant mouse IL-13 (Cat# 413-ML), IL-4 (Cat# 404-ML), IL-2 (Cat# 402-ML) were purchased from R&D Systems. ..

Control:

In Vitro:

Image Search Results

Tumor necrosis factor-like ligand 1A (TL1A) expression and secretion increase as asthma progresses. (A) Western blot analysis of TL1A protein expression in the lung tissues in the wild-type and ovalbumin (OVA)-induced model groups. (B) Changes in the cytokine concentrations of TL1A and immunoglobulin E (IgE) in serum over time following the final challenge in cases and controls. (C) Changes in the cytokine concentrations of TL1A, interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 13 (IL-13), and interferon gamma (IFN-γ) in bronchoalveolar lavage fluid (BAL) over time following the final challenge in cases and controls. (D) Schematic summarizing the timeline of the in vivo asthma modeling with different allergen exposure frequencies. (E) Comparison of total cell counts in the BAL in each group for different challenge numbers. (F) Comparison of IL-13 concentrations in BAL changed for different challenge numbers. (G) Representative plots showing changes in eosinophil counts for different challenge numbers. (H) Changes in IgE concentrations in blood samples for different challenge numbers. (I to K) Representative plots showing Masson staining, hematoxylin and eosin (HE) staining, and periodic acid–Schiff (PAS) staining of pulmonary airway tissue in mice for different challenge numbers. (L) Representative plots showing comparison of S100A4 + CD8 + effector memory T (Tem) cell counts for different challenge numbers. (M) Comparison of TL1A expression level in lung tissue samples from each group. (N and O) Comparison of TL1A concentrations in serum/BAL samples in each group. Data are presented as mean ± SD (error bars) from at least 3 independent experiments, with n = 6 mice per group per experiment (A and E to O). Time-course studies (B and C) used n = 6 mice per time point. * P < 0.05 and ** P < 0.01 compared with the respective control groups. Histological scoring and flow cytometry analysis were performed by investigators blinded to the experimental groups.

Journal: Research

Article Title: Integrated Single-Cell Profiling Reveals TL1A as a Biomarker and Driver of Type 2 Inflammation via Macrophage-Dependent Immunoregulation in Asthma

doi: 10.34133/research.1190

Figure Lengend Snippet: Tumor necrosis factor-like ligand 1A (TL1A) expression and secretion increase as asthma progresses. (A) Western blot analysis of TL1A protein expression in the lung tissues in the wild-type and ovalbumin (OVA)-induced model groups. (B) Changes in the cytokine concentrations of TL1A and immunoglobulin E (IgE) in serum over time following the final challenge in cases and controls. (C) Changes in the cytokine concentrations of TL1A, interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 13 (IL-13), and interferon gamma (IFN-γ) in bronchoalveolar lavage fluid (BAL) over time following the final challenge in cases and controls. (D) Schematic summarizing the timeline of the in vivo asthma modeling with different allergen exposure frequencies. (E) Comparison of total cell counts in the BAL in each group for different challenge numbers. (F) Comparison of IL-13 concentrations in BAL changed for different challenge numbers. (G) Representative plots showing changes in eosinophil counts for different challenge numbers. (H) Changes in IgE concentrations in blood samples for different challenge numbers. (I to K) Representative plots showing Masson staining, hematoxylin and eosin (HE) staining, and periodic acid–Schiff (PAS) staining of pulmonary airway tissue in mice for different challenge numbers. (L) Representative plots showing comparison of S100A4 + CD8 + effector memory T (Tem) cell counts for different challenge numbers. (M) Comparison of TL1A expression level in lung tissue samples from each group. (N and O) Comparison of TL1A concentrations in serum/BAL samples in each group. Data are presented as mean ± SD (error bars) from at least 3 independent experiments, with n = 6 mice per group per experiment (A and E to O). Time-course studies (B and C) used n = 6 mice per time point. * P < 0.05 and ** P < 0.01 compared with the respective control groups. Histological scoring and flow cytometry analysis were performed by investigators blinded to the experimental groups.

Article Snippet: The recombinant proteins, small-molecular compounds, or inhibitors were purchased as indicated: recombinant mouse IL-13, IL-33, and TSLP protein (MCE); recombinant human IL-2, IL-4, TNF-α, and IFN-γ protein (Abbkine); poly(I:C) (Selleck); lipopolysaccharide (Sigma-Aldrich); IL-1β (Thermo Fisher); and small-molecule inhibitors BAY11-7082, LY294002, PD98059, SB203580, and SP600125 (Selleck).

Techniques: Expressing, Western Blot, In Vivo, Comparison, Staining, Control, Flow Cytometry

$Myeloid-cell-specific Tnfsf15 -knockout resulted in the attenuation of allergic airway inflammation. (A) Myeloid-cell-specific Tnfsf15 -knockout strategy. (B) Genotyping was confirmed using tail DNA genomic polymerase chain reaction. (C) Immunomagnetic bead sorting was used to isolate F4/80 + cells, and the expression level of tumor necrosis factor-like ligand 1A (TL1A) was quantified using Western blotting. (D to G) Representative plots showing the proportions of 4 major types of immune cells (macrophages, CD8 + T cells, CD4 + T cells, and B cells) infiltrating the lung tissues in each group. (H) Representative hematoxylin and eosin (HE) staining among the different groups and quantification of the airway inflammation score. Black scale bar, 50 µm. (I) Representative periodic acid–Schiff (PAS) staining among the different groups and quantification of the airway mucus score. (J) Representative Masson’s trichrome staining among the different groups and quantification of the collagen volume fraction. (K and L) Lung eosinophil or neutrophil counts, as determined by flow cytometry. (M) Total cell counts in bronchoalveolar lavage fluid (BAL). (N) Concentrations of interleukin 4 (IL-4) in BAL. (O) Concentrations of interleukin 13 (IL-13) in BAL. Data are presented as mean ± SD. Experiments were repeated 3 times with n = 6 to 8 mice per group per experiment. Cell sorting and Western blot (C) used pooled cells from n = 4 mice. Flow cytometry analyses (D to G and K) and histological scoring (H to J) were performed by investigators blinded to genotype and treatment. * P < 0.05 and ** P < 0.01 compared with the respective groups.$

Journal: Research

Article Title: Integrated Single-Cell Profiling Reveals TL1A as a Biomarker and Driver of Type 2 Inflammation via Macrophage-Dependent Immunoregulation in Asthma

doi: 10.34133/research.1190

Figure Lengend Snippet: Myeloid-cell-specific Tnfsf15 -knockout resulted in the attenuation of allergic airway inflammation. (A) Myeloid-cell-specific Tnfsf15 -knockout strategy. (B) Genotyping was confirmed using tail DNA genomic polymerase chain reaction. (C) Immunomagnetic bead sorting was used to isolate F4/80 + cells, and the expression level of tumor necrosis factor-like ligand 1A (TL1A) was quantified using Western blotting. (D to G) Representative plots showing the proportions of 4 major types of immune cells (macrophages, CD8 + T cells, CD4 + T cells, and B cells) infiltrating the lung tissues in each group. (H) Representative hematoxylin and eosin (HE) staining among the different groups and quantification of the airway inflammation score. Black scale bar, 50 µm. (I) Representative periodic acid–Schiff (PAS) staining among the different groups and quantification of the airway mucus score. (J) Representative Masson’s trichrome staining among the different groups and quantification of the collagen volume fraction. (K and L) Lung eosinophil or neutrophil counts, as determined by flow cytometry. (M) Total cell counts in bronchoalveolar lavage fluid (BAL). (N) Concentrations of interleukin 4 (IL-4) in BAL. (O) Concentrations of interleukin 13 (IL-13) in BAL. Data are presented as mean ± SD. Experiments were repeated 3 times with n = 6 to 8 mice per group per experiment. Cell sorting and Western blot (C) used pooled cells from n = 4 mice. Flow cytometry analyses (D to G and K) and histological scoring (H to J) were performed by investigators blinded to genotype and treatment. * P < 0.05 and ** P < 0.01 compared with the respective groups.

Techniques: Knock-Out, Polymerase Chain Reaction, Expressing, Western Blot, Staining, Flow Cytometry, FACS

Identification of C-C motif chemokine ligand 8 (CCL8) as the key link in the tumor necrosis factor-like ligand 1A (TL1A)-induced allergic inflammatory response. (A) Heatmap showing changes in proallergic chemokine expression levels after gene knockdown. (B) Relative gene expression of CCL8 in lung tissue, quantified using polymerase chain reaction (PCR). (C) Relative gene expression of CCL8 in sorted F4/80 + cell groups in lung tissue, quantified using PCR. (D) CCL8 concentration in bronchoalveolar lavage fluid (BAL) measured using enzyme-linked immunosorbent assay (ELISA). (E) Relative gene expression of CCL8 in lung tissue, quantified using PCR in Tnfsf15 -knockout (KO) mice. (F) CCL8 concentration in BAL measured using ELISA in Tnfsf15 -KO mice. (G) CCL8 expression in the lung tissue of Tnfsf15 transgenic and nontransgenic mice. (H) The correlation between TL1A and CCL8 expression in the Unbiased Biomarkers for the Prediction of Respiratory Disease Outcomes (U-BIOPRED) cohort (based on GSE76262 ). (I) Schematic of the murine ovalbumin-induced asthma models following recombinant TL1A or anti-C-C motif chemokine receptor 8 (anti-CCR8) intervention. (J) Hematoxylin and eosin (HE) staining of lung tissue after allergen challenge and recombinant TL1A (rTL1A) or anti-CCR8 interventions. Black scale bar, 50 µm. (K) Total cell numbers in BAL samples. (L to N) Concentrations of CCL8, interleukin 4 (IL-4), and interleukin 13 (IL-13) in BAL samples from the different groups. Data are presented as mean ± SD from at least 2 independent experiments, with n = 5 to 6 mice per group per experiment. PCR, ELISA, and histological assessments were conducted by researchers blinded to treatment groups. Public dataset correlation (H) used n = samples from the GSE76262 dataset. * P < 0.05 and ** P < 0.01 compared with the respective groups.

Journal: Research

Article Title: Integrated Single-Cell Profiling Reveals TL1A as a Biomarker and Driver of Type 2 Inflammation via Macrophage-Dependent Immunoregulation in Asthma

doi: 10.34133/research.1190

Figure Lengend Snippet: Identification of C-C motif chemokine ligand 8 (CCL8) as the key link in the tumor necrosis factor-like ligand 1A (TL1A)-induced allergic inflammatory response. (A) Heatmap showing changes in proallergic chemokine expression levels after gene knockdown. (B) Relative gene expression of CCL8 in lung tissue, quantified using polymerase chain reaction (PCR). (C) Relative gene expression of CCL8 in sorted F4/80 + cell groups in lung tissue, quantified using PCR. (D) CCL8 concentration in bronchoalveolar lavage fluid (BAL) measured using enzyme-linked immunosorbent assay (ELISA). (E) Relative gene expression of CCL8 in lung tissue, quantified using PCR in Tnfsf15 -knockout (KO) mice. (F) CCL8 concentration in BAL measured using ELISA in Tnfsf15 -KO mice. (G) CCL8 expression in the lung tissue of Tnfsf15 transgenic and nontransgenic mice. (H) The correlation between TL1A and CCL8 expression in the Unbiased Biomarkers for the Prediction of Respiratory Disease Outcomes (U-BIOPRED) cohort (based on GSE76262 ). (I) Schematic of the murine ovalbumin-induced asthma models following recombinant TL1A or anti-C-C motif chemokine receptor 8 (anti-CCR8) intervention. (J) Hematoxylin and eosin (HE) staining of lung tissue after allergen challenge and recombinant TL1A (rTL1A) or anti-CCR8 interventions. Black scale bar, 50 µm. (K) Total cell numbers in BAL samples. (L to N) Concentrations of CCL8, interleukin 4 (IL-4), and interleukin 13 (IL-13) in BAL samples from the different groups. Data are presented as mean ± SD from at least 2 independent experiments, with n = 5 to 6 mice per group per experiment. PCR, ELISA, and histological assessments were conducted by researchers blinded to treatment groups. Public dataset correlation (H) used n = samples from the GSE76262 dataset. * P < 0.05 and ** P < 0.01 compared with the respective groups.

Techniques: Expressing, Knockdown, Gene Expression, Polymerase Chain Reaction, Concentration Assay, Enzyme-linked Immunosorbent Assay, Knock-Out, Transgenic Assay, Recombinant, Staining

A. volcano plot of discovery metabolomics comparing BMDMs that stimulated with LPS/IFNγ (at 48h post stimulation) versus unstimulated. Colored points indicate parent feature (blue circle) and 34 S isotope peak candidates (purple circle) with orange line indicated parent-isotope pair. Only parent feature that meet > 2.5-fold change and significance < 0.05 are labeled. Citrulline (green triangle) (and its isotope peaks and MS adducts) are labeled as a positive control for classical activation induced metabolites. Each point represents the mean of n = 3 biological replicates. B. volcano plot of metabolomic features in parallel cells cultured in media containing unlabeled cystine versus 3,3- 13 C 2 -L-cystine. Pairs with significant isotopic shift are colored blue and red. Pairs that match parent features in (A) are labeled with m/z values. Each point represents the mean, n = 3 biological replicates. C. Extracted ion chromatograms (EICs) for major cysteine adducts in LPS/IFNγ-stimulated BMDM (red) compared to in unstimulated BMDMs (blue). Identified molecular formulas based on isotopic distribution and exact mass are labeled. Formulas shown in red do not correspond to known mammalian metabolites. D-F. The changes in major cysteine-adducts in BMDMs over a time course of LPS/IFNγ stimulation (blue) versus IL-4/IL-13 stimulation (green). Mean +/- standard deviation, n= 3 biological replicates.

Journal: bioRxiv

Article Title: Discovery of metabolites produced by reactions between central carbon metabolites and cysteine that mark inflammatory macrophages

doi: 10.64898/2026.03.18.712640

Figure Lengend Snippet: A. volcano plot of discovery metabolomics comparing BMDMs that stimulated with LPS/IFNγ (at 48h post stimulation) versus unstimulated. Colored points indicate parent feature (blue circle) and 34 S isotope peak candidates (purple circle) with orange line indicated parent-isotope pair. Only parent feature that meet > 2.5-fold change and significance < 0.05 are labeled. Citrulline (green triangle) (and its isotope peaks and MS adducts) are labeled as a positive control for classical activation induced metabolites. Each point represents the mean of n = 3 biological replicates. B. volcano plot of metabolomic features in parallel cells cultured in media containing unlabeled cystine versus 3,3- 13 C 2 -L-cystine. Pairs with significant isotopic shift are colored blue and red. Pairs that match parent features in (A) are labeled with m/z values. Each point represents the mean, n = 3 biological replicates. C. Extracted ion chromatograms (EICs) for major cysteine adducts in LPS/IFNγ-stimulated BMDM (red) compared to in unstimulated BMDMs (blue). Identified molecular formulas based on isotopic distribution and exact mass are labeled. Formulas shown in red do not correspond to known mammalian metabolites. D-F. The changes in major cysteine-adducts in BMDMs over a time course of LPS/IFNγ stimulation (blue) versus IL-4/IL-13 stimulation (green). Mean +/- standard deviation, n= 3 biological replicates.

Article Snippet: For alternative activation, BMDMs were treated with with 20 ng ml −1 IL-4 (cat. no. 404-ML, R&D Systems) and 20 ng ml −1 IL-13 (cat. no. 413-ML-005, R&D Systems).

Techniques: Labeling, Positive Control, Activation Assay, Metabolomic, Cell Culture, Standard Deviation

Review

recombinant mouse il 13 (R&D Systems)

Structured Review

Images

Related Articles

Recombinant:

Control:

In Vitro:

Similar Products

Image Search Results